Selenicereus undatus, commonly known as dragon fruit, is widely consumed for its nutritional benefits and potential medicinal properties. This study explores the hemolytic activity of dragon fruit extracts to evaluate their impact on red blood cells. Aqueous and ethanolic extracts of S. undatus were prepared and tested for hemolytic effects on human erythrocytes using hemoglobin release assays. Results revealed that both extracts displayed minimal hemolysis at lower concentrations (≤1 mg/mL), suggesting limited cytotoxicity. However, at higher concentrations (≥5 mg/mL), the ethanolic extract showed significant hemolytic activity, indicating potential erythrocyte membrane damage. These findings highlight the need for caution in consuming high doses of dragon fruit extracts, although the low concentrations typically found in dietary selenicereus undatus, Organic extracts, hemolytic activity, therapeutic plants, and phytochemical screeninuse appear safe.