In the Indian medical system, Ayurveda, piper betle and Ocimum tenuiflorum, has been described as a therapy for several infectious infections and maladies. The overall function of several active ingredients is what determines the effectiveness of herbal medicines since these ingredients work in concert to increase the therapeutic value of the product. The present study objective was to create and assess a multiherbal gel combining liquid piper betle extract as well as Ocimum tenuiflorum based on the folklore use. Gel formulations (A and B) were created using ethanolic extracts of Ocimum tenuiflorum and Piper betle at concentrations in a base. It was found that the polyherbal gel compositions have antibacterial properties. The formulation’s plant ingredients synergistic action may be responsible for the effective activity. Numerous actions, including antioxidant, antidiabetic, chemo preventive, anti-ulcer, and immunological response regulation, have been reported for Ocimum tenuiflorum. It is well known that piper betles contain a number of medicinal qualities, including antibacterial, hypoglycemic, antifungal, and antioxidant effects.