The current study used in vitro methods to analyze the effects of Epiphyllum Oxypetalum extract on calcium oxalate. The leaves of Epiphyllum Oxypetalum were extracted using the Soxhlet extraction method using a solvent such as ethanol. The extracted alkaloid, flavonoid, saponin, phenol, and terpenoid content, as well as other phytochemicals, were all determined. For the in-vitro study, experimental calcium oxalate stones were generated and compared to standard treatment. Cystone is administered as normal. It has a high capacity for calcium oxalate dissolution and is high in phytochemical such as alkaloids, saponin, glycosides, terpenoids, and flavonoids. These flavonoids prevent the formation containing calcium oxalate deposits in the kidney tubules. The leaf extract contains anti-urolithiasis therapeutic and preventive capabilities, as well as the capability of reducing the size of stones. In addition to diuretic and antiurolithic actions, it has anti-diabetic, anti-cancer, anti-ulcer, anti-microbial, and wound healing qualities. The primary goal of the research is to investigate how the plant Epiphyllum Oxypetalum, namely its leaves, can be used to prevent and treat health problems such as kidney stones, which are increasingly common in younger people due to inactivity and poor diet. Because the extract may eliminate small particles from the kidney and urinary system, they are less likely to become stuck in the urinary tract and form stones.