People are increasingly turning to medicinal plants due to their many benefits. Anything made using herbs are frequently accepted in the treatment of many ailments. While many of these claims may be considered contradictory, it is acknowledged that over 80% of the world’s population is addicted herbs and products to keep their health clean. However, the increased use of herbal products also leads to abuse and adulteration, causing tension between consumers and producers, sometimes with fatal consequences. The development of reliable assays that can enable the exact determination of Including phytochemicals the composition of markers/bioactive compounds and other important factors, poses significant challenges for researchers. Standardization production of herbal medicines, establishing uniform biological activity, uniform dosage or quality assurance services. This review article discusses various techniques and recent advances such as DNA fingerprinting, metabolomics methods, differential pulse polarography, chemometrics, and X-ray diffraction. Additionally, the contribution of capillary electrophoresis and chromatography techniques in medicinal plant modeling has also been reported.