The plant Selenicereus undatus, sometimes referred to as dragon fruit, has a variety of bioactive substances that have been shown to have pharmacological effects. Recurrence rates and related comorbidities continue to be major obstacles in spite of therapy improvements. Because of their possible therapeutic advantages in the management of urolithiasis, natural products have drawn attention. The purpose of this work was to assess, using experimental models, the anti-urolithiatic potential of S.undatus extract. In order to identify the bioactive compounds that make up the ethanolic extract of S. undatus, a phytochemical screening procedure was conducted. Following the administration of S. undatus extract to the animals, many parameters were examined, including urine pH, urinary biochemical components, kidney histology, and stone development.