Nanotechnology has revolutionized pharmaceutical analysis through innovative methods in drug delivery, diagnostics, and quality assurance. This review explores various nanomaterials, including lipid-based, polymeric, and metallic nanoparticles, and their diverse applications in enhancing analytical techniques such as spectroscopy, chromatography, and nanosensing.Improved sensitivity and selectivity in detection techniques, multipurpose platforms for concurrent drug delivery and imaging, and targeted medicines that reduce adverse effects while optimizing therapeutic efficacy are some of the main potential offered by nanotechnology. Nanotechnology also offers the possibility of cost-effective approaches to drug analysis and development. Nevertheless, there are certain difficulties in using nanotechnology into pharmaceutical analysis. Concerns about safety and toxicity associated with the use of nanomaterials, as well as the dynamic character of nanomedicine, provide significant regulatory challenges. Additionally, the absence of defined procedures makes quality control more difficult and raises concerns about the efficacy and uniformity of product formulation. Obstacles that must be overcome include public opinion and acceptance of nanotechnology in healthcare.