Tropical developing nations have higher rates of skin illnesses because of mycotic diseases. Herbal medicine has long been used in many regions of the world to treat skin-related issues, including mycotic infections. The medications used for the treatment of dermatophytosis have a number of adverse effects and possess a restricted level of effectiveness. Thus, the development of novel, safer, and more potent antifungal in situ gel is clearly needed. Since the relative toxicity of antifungal in situ gel and their increasing resistance to them are issues that traditional herbal medicine may be able to address with the use of herbal therapies. This study used dilution agar to assess the in-vitro antifungal efficacy of a leaf extract from Calotropis gigantea against three distinct genera of dermestids: Microscopium, Trichophyton, and Epidermophyton. In this study, an in-situ gel formulation containing Calotropis gigantea extract was developed and evaluated for its potential in treating fungal infections. The antifungal activity of the in-situ gel formulation was assessed using the agar diffusion assay against selected fungal strains. The formulation demonstrated significant antifungal activity, with zones of inhibition comparable to standard antifungal agents.