Zornia gibbosa, a plant species known for its medicinal properties, has been traditionally used in various folk medicines. Earthworms were used as a model organism in this study to assess the antihelmintic efficacy of Z. gibbosa extract. Earthworms are widely employed in pharmacological research due to their physiological similarity to higher organisms and sensitivity to various substances. The extract of Z. gibbosa was prepared using standard extraction methods, and its antihelmintic activity was assessed using Eisenia fetida earthworms. To ascertain the dose-dependent response, the extract was tested at various concentrations. The length of time it took for the earthworms to become paralyzed and die after being exposed to the extract were among the factors that were assessed. The results revealed a significant dose-dependent antihelmintic activity of Z. gibbosa extract against earthworms. The extract exhibited a rapid onset of paralysis and mortality in the treated earthworms compared to the control group. These findings suggest the potential of Z. gibbosa as a source of natural antihelmintic agents. Overall, this research contributes to the exploration of natural alternatives for controlling helminthic infections, addressing the growing concern of drug resistance and adverse effects associated with synthetic anthelmintic drugs.