This study evaluated Zornia gibbosa span extracts anti-urolithiatic efficacy in vitro. Flavonoids, alkaloids, and tannins were detected in the extract, which was made with ethanol as the solvent, according to a qualitative phytochemical examination. In-vitro assays were conducted to evaluate the extracts potential in inhibiting urinary crystal formation, preventing crystal aggregation, and reducing oxalate levels. Additionally, antioxidant activity was assessed using DPPH scavenging assay. The extract exhibited significant inhibition of crystal formation and aggregation, along with notable antioxidant activity. This research highlights the potential of Zornia gibbosa span as a natural anti-urolithiatic agent, contributing to the development of novel therapeutic strategies for urolithiasis. In-vitro assays were conducted to assess its ability to inhibit urinary crystal formation and aggregation, reduce oxalate levels, and scavenge free radicals. The extract demonstrated significant inhibition of crystal formation and aggregation, accompanied by notable antioxidant activity. These findings suggest the potential of Zornia gibbosa span as a natural remedy for urolithiasis. Its mechanism of action and in vivo efficacy need to be further investigated in order to facilitate the creation of innovative urolithiasis treatment strategies.