Urolithiasis, affects millions worldwide, causing severe pain and potential renal damage. Traditional treatments include invasive surgical procedures and pharmacological therapies, which often have limitations such as high costs, side effects, and recurrence of stones. Consequently, there is a growing interest in exploring natural remedies with fewer side effects and cost-effective properties. Vachellia eburnea, a plant known for its medicinal properties in traditional medicine, particularly in treating urinary disorders, offers a promising alternative Urolithiasis, a significant global health problem, involves the formation of stones in the urinary system, primarily composed of calcium oxalate. The condition can cause severe pain, urinary obstruction, and potential renal damage. Conventional treatments include surgical intervention and pharmacotherapy, which often carry risks of recurrence, high costs, and adverse side effects. Therefore, there is an increasing demand for alternative therapies derived from natural sources. Vachellia eburnea, traditionally used in folk medicine for treating various ailments, including urinary disorders, presents a promising candidate for exploring its anti-urolithiatic potential.