Gulandina bonduc, also known as Caesalpinia bonduc or Gray Nicker, is a plant species with a rich history of traditional medicinal use. This review focuses on the in vitro hemolytic activity research work conducted on Gulandina bonduc. Hemolysis is the process of red blood cell destruction, which can have implications for various health conditions.Research studies have investigated the potential hemolytic activity of Gulandina bonduc extracts and isolated compounds. The findings suggest that certain components of the plant may exhibit hemolytic properties in vitro. These studies have utilized red blood cell models to assess the impact of Gulandina bonduc on cell membrane integrity and hemoglobin release. Understanding the hemolytic activity of Gulandina bonduc is crucial for evaluating its safety profile and potential therapeutic applications. Further research is needed to elucidate the specific bioactive compounds responsible for the observed hemolytic effects and to determine the mechanisms underlying these activities. Overall, investigating the in vitro hemolytic activity of Gulandina bonduc contributes to the comprehensive understanding of its pharmacological properties and aids in assessing its suitability for therapeutic use. Additional studies are warranted to explore the implications of these findings in the context of human health and disease management.