When assessing the efficacy, safety, and quality of plant-based final products such as medicines, nutraceuticals and herbal supplements phytochemical analysis is essential. Because of their great sensitivity, specificity and capacity to separate intricate mixtures of phytochemicals, chromatographic techniques in particular, Thin Layer Chromatography (TLC) Gas Chromatography (GC), and great-Performance Liquid Chromatography (HPLC) re frequently utilized for this purpose. The use of these methods in the qualitative and quantitative evaluation of phytochemicals such as terpenes, polyphenols, alkaloids, and flavonoids in final goods is examined in this work. Because HPLC is so accurate at quantifying individual molecules, it is often used; nevertheless, GC works especially well with volatile compounds, including essential oils. TLC is still a cost-effective method for screening phytochemical profiles at an early stage. The analysis also covers Issues such matrix effects, sample preparation, and the requirement for established analytical techniques to guarantee dependability and repeatability in phytochemical analysis. The study emphasizes how crucial it is to use chromatographic techniques to guarantee the therapeutic potential, consistency, and quality of completed plant-based products, thereby promoting consumer safety and regulatory compliance.