Even with global biodefense efforts, the issue of very dangerous pathogen dissemination persists, particularly in light of the possibility of terrorist attacks. Thus, a current issue is the discovery and development of novel, highly effective antimicrobial compounds that are effective against a broad spectrum of highly pathogenic microbes, which are the cause of the majority of deadly diseases that affect both humans and animals. Metal nanoparticles have a great deal of promise in this field. The primary objective is to examine the bismuth nanoparticles antimicrobial efficacy against the pathogenic bacteria Staphylococcus aureus and the opportunistic, dimorphic yeast Candida albicans. against the opportunistic, dimorphic yeast Candida albicans and the harmful bacteria Staphylococcus aureus. Both the dimorphic yeast strain C. albicans and the methicillin-sensitive strain of S. aureus, A Gram-positive bacterium, were employed. S. aureus and C. albicans were inoculated in tryptic soy broth (TSB) and yeast extract peptone Dextrose (YPD) broth, respectively, and were incubated for growth for the entire night at 35 °C in an orbital shaker. Bismuth nanoparticles work against microorganisms in a number of ways. They increase the generation of reactive oxygen species, which puts microbial cells under oxidative stress. This damages DNA and membranes, among other biological components. They can also prevent the formation of biofilms by lowering adhesion and interfering with signalling pathways. The samples were examined using scanning electron microscopy (SEM) to determine the effect of the bismuth nanoparticles affects S. aureus and Candida albicans cell shape and biofilm structure. Furthermore, the antimicrobial activity of bismuth nanoparticles is comparable in strength to that of silver nanoparticles and parallels that of widely available medicines. Additional research on the biocompatibility and safety of bismuth nanoparticles is necessary for future applications, as evidenced by literature suggesting that these particles may exhibit cytotoxicity.